Overview
| Product name | Recombinant Human NKG-2D/KLRK1/CD314 Protein |
|---|
| Catalog No. | RP01879 |
|---|
| Description | Recombinant Human NKG-2D/KLRK1/CD314 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (ILe73-Val216) of Human NKG-2D/KLRK1/CD314 (Accession #NP_001186734.1) fused with a hFc
at the N-terminus. |
|---|
| Bio-Activity | Measured by its binding ability in a functional ELISA. Immobilized Human MICA(Catalog: RP00172) at 2 μg/mL (100 μL/well) can bind Human NKG-2D/KLRK1/CD314 (Catalog: RP01879) with a linear range of 0.005-0.98 μg/mL.
|
|---|
| Endotoxin | <0.1EU/μg of the protein by LAL method. |
|---|
| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
|---|
| Species | human |
|---|
| Calculated MW | 42.61 kDa |
|---|
Background
NKG2D is a transmembrane protein belonging to the CD94/NKG2 family of C-type lectin-like receptors, also known as KLRK1, CD314, D12S2489E, KLR and killer cell lectin like receptor K1. NKG2D itself forms a homodimer whose ectodomains serve for ligand binding. NKG2D is a major recognition receptor for the detection and elimination of transformed and infected cells as its ligands are induced during cellular stress, either as a result of infection or genomic stress such as in cancer. In NK cells, NKG2D serves as an activating receptor, which itself is able to trigger cytotoxicity. The function of NKG2D on CD8+ T cells is to send co-stimulatory signals to activate them.
Product information
| Synonym | KLR; CD314; NKG2D; NKG2-D |
|---|
| Expression Host | HEK293 cells |
|---|
| Tag | N-hFC |
|---|
| Swiss-Prot | |
|---|
| Gene ID | |
|---|
Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
|---|
| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
|---|
* For research use only. Not for therapeutic or diagnostic purposes.
