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Recombinant Human NSE/ENO2 Protein (RP02827)

Datasheet

Overview

Product nameRecombinant Human NSE/ENO2 Protein
Catalog No.RP02827
DescriptionRecombinant Human NSE/ENO2 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Leu434) of human NSE/ENO2 (Accession #NP_001966.1) fused with a 6×His tag at the N-terminus.
Purity> 80% as determined by by reducing SDS-PAGE.
Endotoxin<1EU/μg
FormulationLyophilized from a 0.22 μm filtered solution of 20mM Tris-HCl, 100mM KCl, 5mM MgSO4, pH 7.5.
SpeciesHuman
Calculated MW55 KDa
Gamma-enolase, also known as Enolase 2, belongs to the enolase family. The alpha/alpha homodimer of ENO2 is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells. Levels of ENO2 increase dramatically in cardiovascular accidents, cerebral trauma, brain tumors and Creutzfeldt-Jakob disease. ENO2 has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. It binds to cultured neocortical neurons and promotes cell survival in a calcium-dependent manner.
SynonymGamma-enolase; 2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase; NSE; ENO2
Expression HostE. coli
TagN-His
Swiss-Prot
Gene ID
StorageStore at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
ReconstitutionCentrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.

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