| Product name | Recombinant Human Neurotrophin-3/NGF-2/NT-3 Protein |
|---|---|
| Catalog No. | RP00496 |
| Description | Recombinant Human Neurotrophin-3/NGF-2/NT-3 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Tyr139-Thr257) of Human Neurotrophin-3/NGF-2/NT-3 (Accession # P20783) fused with No-Tag. |
| Bio-Activity | Measured by its binding ability in a functional ELISA.Immobilized Recombinant Human TrkB (Catalog: RP00290) at 2μg/mL (100 μL/well) can bind Recombinant Human Neurotrophin-3/NGF-2/NT-3 with a linear range of 1.95-35.5 ng/mL. |
| Purity | ≥ 95 % as determined by SDS-PAGE. |
| Endotoxin | < 0.01 EU/μg of the protein by LAL method |
| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 5.2. |
| Species | Human |
| Calculated MW | 13.6 kDa |
| Synonym | NTF3; Neurotrophin-3; NT-3; HDNF; Nerve growth factor 2; NGF-2; Neurotrophic factor |
|---|---|
| Expression Host | E. coli |
| Tag | No-Tag |
| Swiss-Prot | |
| Gene ID |
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
|---|---|
| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
* For research use only. Not for therapeutic or diagnostic purposes.
Publishing research using RP00496? Please let us know so that we can cite the reference in this datasheet.
