Overview
| Product name | Recombinant Human PDGF-BB Protein |
|---|
| Catalog No. | RP01763 |
|---|
| Description | Recombinant Human PDGF-BB Protein is produced by Pichia expression system. The target protein is expressed with sequence (Ser82-Thr190) of human PDGF-BB (Accession #NP_002599.1) fused with a additional amino acid free. |
|---|
| Bio-Activity | 1.Immobilized PDGF-BB (Catalog: RP01763) at 2 μg/mL (100 μL/well) can bind Human PDGFRB (Catalog: RP00126) with a linear range of 0.1-75.4 ng/mL.2.Recombinant Human PDGF-BB Protein was measured in a cell proliferation assay using BALB/3T3 mouse fibroblasts. The ED50 for this effect is 2.09-8.36 ng/mL, corresponding to a specific activity of 1.20×105~4.78×105 units/mg.
|
|---|
| Endotoxin | < 0.1EU/μg |
|---|
| Formulation | Lyophilized from a 0.22 μm filtered solution of 20mM NaAc，pH 4.5. |
|---|
| Species | Human |
|---|
| Calculated MW | 12.30 kDa |
|---|
Background
Platelet-derived growth factor-B (PDGFB) is necessary for normal cardiovascular development. The administration of PDGFB alone normalized tumor vasculature by increasing periendothelial coverage and vascular functionality. Interestingly, this effect exerted by PDGFB was also observed in the presence of DAPT. So PDGFB is able to improve tumor vascularity and allows the anticancer action of DAPT in the tumor.
Product information
| Synonym | SIS; SSV; IBGC5; PDGF2; c-sis; PDGF-2; PDGF subunit B; PDGF-BB; PDGFB |
|---|
| Expression Host | Pichia |
|---|
| Tag | No-tag |
|---|
| Swiss-Prot | |
|---|
| Gene ID | |
|---|
Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
|---|
| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
|---|
* For research use only. Not for therapeutic or diagnostic purposes.
