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Recombinant Human PSA/KLK3 Protein (RP01540)

Bioactivityvalidated

Datasheet

Overview

Product nameRecombinant Human PSA/KLK3 Protein
Catalog No.RP01540
DescriptionRecombinant Human PSA/KLK3 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala18-Pro261) of human PSA/KLK3 (Accession #NP_001639.1) fused with a 6×His tag at the C-terminus.
Bio-ActivityMeasured by its ability to cleave the colorimetric peptide substrate, Succinyl-Arg-Pro-Tyr-p- Nitroanilide (Suc-RPY-pNA). The specific activity is >160 pmol/min/μg, as measured under the described conditions.
Purity> 95% by SDS-PAGE.
Endotoxin<0.1EU/μg
FormulationLyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
SpeciesHuman
Calculated MW27.68 kDa
KLK3 (Kallikrein Related Peptidase 3) is a Protein Coding gene. The gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. It encodes a single-chain glycoprotein, a protease that is synthesized in the epithelial cells of the prostate gland and is present in seminal plasma. KLK3, also known as Prostate Specific Antigen (PSA), kallikrein-related peptidase 3, Gamma-seminoprotein, is a secreted protein of the glandular kallikrein subfamily of serine proteases. KLK3 contains one peptidase S1 domain. KLK3 is a glycoprotein produced almost exclusively by the prostate gland. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers.
SynonymAPS; KLK2A1; PSA; hK3; KLK3; APS; prostate-specific antigen; KLK2A1; PSA; hK3
Expression HostHEK293 cells
TagC-His
Swiss-Prot
Gene ID
StorageStore at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
ReconstitutionCentrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.

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