Overview
Product name | Recombinant Human PSA/KLK3 Protein |
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Catalog No. | RP01540 |
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Description | Recombinant Human PSA/KLK3 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala18-Pro261) of human PSA/KLK3 (Accession #NP_001639.1) fused with a 6×His tag at the C-terminus. |
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Bio-Activity | Measured by its ability to cleave the colorimetric peptide substrate, Succinyl-Arg-Pro-Tyr-p- Nitroanilide (Suc-RPY-pNA). The specific activity is >160 pmol/min/μg, as measured under the described conditions.
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Purity | > 95% by SDS-PAGE. |
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Endotoxin | <0.1EU/μg |
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Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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Species | Human |
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Calculated MW | 27.68 kDa |
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Background
KLK3 (Kallikrein Related Peptidase 3) is a Protein Coding gene. The gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. It encodes a single-chain glycoprotein, a protease that is synthesized in the epithelial cells of the prostate gland and is present in seminal plasma. KLK3, also known as Prostate Specific Antigen (PSA), kallikrein-related peptidase 3, Gamma-seminoprotein, is a secreted protein of the glandular kallikrein subfamily of serine proteases. KLK3 contains one peptidase S1 domain. KLK3 is a glycoprotein produced almost exclusively by the prostate gland. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers.
Product information
Synonym | APS; KLK2A1; PSA; hK3; KLK3; APS; prostate-specific antigen; KLK2A1; PSA; hK3 |
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Expression Host | HEK293 cells |
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Tag | C-His |
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Swiss-Prot | |
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Gene ID | |
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Usage guide
Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.