Overview
| Product name | Recombinant Human Plasma Kallikrein/KLKB1 Protein |
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| Catalog No. | RP02872 |
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| Description | Recombinant Human Plasma Kallikrein/KLKB1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly20-Ala638) of human Plasma Kallikrein/KLKB1 (Accession #NP_000883.2) fused with 6×His tag at the C-terminus. |
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| Purity | > 95% as determined by SEC-HPLC. |
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| Endotoxin | <1EU/μg |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of 20mM NaAc, 150mM NaCl, pH5.0. |
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| Species | Human |
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| Calculated MW | 70.3 kDa |
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Background
Plasma kallikrein, also known as Fletcher factor or kallikrein B1 (KLKB1), is a serine endopeptidase, like its homologs tissue kallikrein and kallikrein-related peptidases (KLKs). Its physiological role is to catalyze the release of kinins and other vasoactive peptides.
Product information
| Synonym | KLKB1; KLK3; PKK; PKKD; PPK |
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| Expression Host | HEK293 cells |
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| Tag | C-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in 20mM NaAc, 150mM NaCl, pH5.0.. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
