| Product name | Recombinant Human RNF43 Protein |
|---|---|
| Catalog No. | RP02777 |
| Description | Recombinant Human RNF43 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly24-Tyr197) of human RNF43 (Accession #NP_001292473.1) fused with a hFc tag at the C-terminus. |
| Bio-Activity | 1.Immobilized Human R spondin 1, His Tag at 5 μg/mL (100μL/well) on the plate. Dose response curve for Human RNF34, hFc Tag with the EC50 of 0.4 μg/mL determined by ELISA. 2.Immobilized Human R-Spondin 3, His Tag at 1 μg/mL (100μL/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 16.1 ng/mL determined by ELISA. |
| Purity | > 95% as determined by HPLC |
| Endotoxin | <1EU/μg |
| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Species | Human |
| Calculated MW | 45.8 kDa |
| Synonym | URCC; SSPCS; RNF124 |
|---|---|
| Expression Host | HEK293 cells |
| Tag | C-hFC |
| Swiss-Prot | |
| Gene ID |
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
|---|---|
| Reconstitution | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
* For research use only. Not for therapeutic or diagnostic purposes.
