| Product name | Recombinant Human R-spondin-1/RSPO1 Protein |
|---|---|
| Catalog No. | RP00071 |
| Description | Recombinant Human R-spondin-1/RSPO1 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Arg31-Ala263) of human R-Spondin1 (Accession #NP_001033722.1) fused with a 6×His tag at the C-terminus. |
| Bio-Activity | 1.Measured by its ability to enhance Cyclin D1 expression in HCT116 human colon adenocarcinoma cells. 0.1-10ng/mL of Recombinant Human RSPO1 can effectively enhance Cyclin D1 expression. 2.The intestinal crypts of mice were cultured in organoid culture medium containing factor combinations (100 ng/mL Noggin, Cat. RP01237 + 500 ng/mL R-spindin-1, Cat. RP00071) derived from ABclonal for144 hours, intestinal organoids were formed. (Customer Feedback Data) 3.Recombinant Human R-Spondin 1 protein stimulated Wnt signal pathway with Wnt-3a protein in HEK293T cells. After 6 hours, the stimulation when adding 300 ng/mL of R-Spondin-1 reached highest effect. Compared with only Wnt-3a stimulation, the Wnt signaling pathway was enhanced 3.1-fold after adding 300 ng/mL R-Spondin 1. |
| Purity | > 95% by SDS-PAGE. |
| Endotoxin | < 0.1 EU/μg of the protein by LAL method. |
| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| Species | Human |
| Calculated MW | 26.44 kDa |
| Synonym | RSPO1; CRISTIN3; RSPO |
|---|---|
| Expression Host | HEK293 cells |
| Tag | C-His |
| Swiss-Prot | |
| Gene ID |
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
|---|---|
| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
* For research use only. Not for therapeutic or diagnostic purposes.
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