Overview
| Product name | Recombinant Human Semaphorin-5A/SEMA5A Protein |
|---|
| Catalog No. | RP01093 |
|---|
| Description | Recombinant Human Semaphorin-5A/SEMA5A Protein is produced by Mammalian expression system. The target protein is expressed with sequence (Glu23-Thr765) of human Semaphorin-5A (Accession #Q13591) fused with a 6×His tag at the C-terminus. |
|---|
| Purity | > 95% by SDS-PAGE. |
|---|
| Endotoxin | < 1.0 EU/μg of the protein by LAL method. |
|---|
| Formulation | Lyophilized from a 0.22 μm filtered solution of 20mM PB, 150mM NaCl, 0.1mM EDTA, 0.05% Tween 20, pH 7.2.Contact us for customized product form or formulation. |
|---|
| Species | Human |
|---|
| Calculated MW | 84.71 kDa |
|---|
Background
This protein is a mbrane protein containing a semaphorin domain and several thrombospondin type-1 repeats. Members of this family are involved in axonal guidance during neural development. This gene has been implicated as an autism susceptibility gene.
Product information
| Synonym | SEMA5A; SEMAF; semF |
|---|
| Expression Host | HEK293 cells |
|---|
| Tag | C-His |
|---|
| Swiss-Prot | |
|---|
| Gene ID | |
|---|
Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
|---|
| Reconstitution | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
|---|
* For research use only. Not for therapeutic or diagnostic purposes.
