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Recombinant Human Serpin C1/Antithrombin-III Protein (RP00140)

Bioactivityvalidated

Publication (1) Datasheet

Overview

Product nameRecombinant Human Serpin C1/Antithrombin-III Protein
Catalog No.RP00140
DescriptionRecombinant Human SerpinC1/AntithrombinIII/ATIII Protein is produced by HEK293 expression system. The target protein is expressed with sequence (His33-Lys464) of human SerpinC1/AntithrombinIII/ATIII (Accession #NP_000479.1) fused with a 6×His tag at the C-terminus.
Bio-ActivityMeasured by its ability to inhibit Recombinant Human Coagulation Factor II/Thrombin cleavage of a fluorogenic peptide substrate Boc-VPR-AMC. The IC50 value is <0.48 nM.
Purity> 95% by SDS-PAGE.
Endotoxin< 0.1 EU/μg of the protein by LAL method.
FormulationLyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
SpeciesHuman
Calculated MW49.88 kDa
The protein is a plasma protease inhibitor and a member of the serpin superfamily. This protein inhibits thrombin as well as other activated serine proteases of the coagulation system, and it regulates the blood coagulation cascade. The protein includes two functional domains: the heparin binding-domain at the N-terminus of the mature protein, and the reactive site domain at the C-terminus. The inhibitory activity is enhanced by the presence of heparin. More than 120 mutations have been identified for this gene, many of which are known to cause antithrombin-III deficiency.
SynonymAT3; AT3D; ATIII; THPH7; SERPINC1; AT3D; ATIII; THPH7
Expression HostHEK293 cells
TagC-His
Swiss-Prot
Gene ID
StorageStore at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
ReconstitutionCentrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using RP00140? Please let us know so that we can cite the reference in this datasheet.

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