Recombinant Human Siglec-10 Protein (RP02498)

Overview

Product name	Recombinant Human Siglec-10 Protein
Catalog No.	RP02498
Description	Recombinant Human Siglec 10 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Met17-Thr546) of Human Siglec 10 fused with hFc tag at the C-terminal.
Purity	> 95% by Tris-Bis PAGE
Endotoxin	< 0.1 EU/μg
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Species	Human
Calculated MW	84.18 kDa

Siglec-10 is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. It is also reported to bind to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24 also known as HSA (Heat-stable antigen).Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system.

Product information

Synonym	SLG2; PRO940; SIGLEC-10; SIGLEC10
Expression Host	HEK293 cells
Tag	C-hFc
Swiss-Prot
Gene ID

Usage guide

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.