Overview
| Product name | Recombinant Human Siglec-15/CD33L3 Protein |
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| Catalog No. | RP01256 |
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| Description | Recombinant Human Siglec-15/CD33L3 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Phe20-Thr263) of human Siglec-15/CD33L3 (Accession #NP_998767.1) fused with a 6×His tag at the C-terminus. |
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| Bio-Activity | Measured by its ability to inhibit Anti-CD3-induced proliferation of jurkat cells. The ED50 for this effect is 4.99-19.98 μg/mL.
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| Purity | >95% by SDS-PAGE. |
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| Endotoxin | < 1.0 EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, 300mM NaCl, pH 7.4.Contact us for customized product form or formulation. |
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| Species | Human |
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| Calculated MW | 27.40 kDa |
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Product information
| Synonym | CD33L3; HsT1361; SIGLEC-15; SIGLEC15; Siglec-15 |
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| Expression Host | HEK293 cells |
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| Tag | C-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
