Overview
| Product name | Recombinant Human TIE2/TEK/CD202b Protein |
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| Catalog No. | RP00048 |
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| Description | Recombinant Human TIE2/TEK/CD202b Protein is produced by insect cell-baculovirus expression system. The target protein is expressed with sequence (Lys808-Ala1124) of human hTIE2 (Accession #NP_000450.2). |
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| Bio-Activity | Measured by its binding ability in a functional ELISA. Immobilized Human TEK Protein at 2 μg/mL (100 μL/well) can bind ANG2 with a linear range of 39-7090 ng/mL.
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| Purity | > 97% by SDS-PAGE. |
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| Endotoxin | < 1.0 EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
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| Species | Human |
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| Calculated MW | 36.21 kDa |
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Background
Tyrosine-protein kinase that acts as cell-surface receptor for ANGPT1, ANGPT2 and ANGPT4 and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Has anti-inflammatory effects by preventing the leakage of proinflammatory plasma proteins and leukocytes from blood vessels. Required for normal angiogenesis and heart development during embryogenesis. Required for post-natal hematopoiesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts.
Product information
| Synonym | TEK; CD202B; GLC3E; TIE-2; TIE2; VMCM; VMCM1; Tie2 |
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| Expression Host | Baculovirus-Infected Sf9 Cells |
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| Tag | No tag |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
