Recombinant Human TIM-3/HAVCR2/CD366 Protein (RP00234)

Overview

Product name	Recombinant Human TIM-3/HAVCR2/CD366 Protein
Catalog No.	RP00234
Description	Recombinant Human TIM-3/HAVCR2/CD366 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ser22-Arg200 ) of human TIM-3 (Accession #NP_116171.3) fused with an Fc, 6×His tag at the C-terminus.
Bio-Activity	Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Galectin9 at 2 μg/mL can bind recombinant human HAVCR2 with a linear range of 0.3-5 μg/mL.
Purity	> 87% by SDS-PAGE.
Endotoxin	< 0.1 EU/μg of the protein by LAL method.
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Species	Human
Calculated MW	46.66 kDa

HAVCR2 also known as TIM3(T cell immunoglobulin and mucin domain-3), is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk region. Mature human TIM-3 consists of a 181 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 78 aa cytoplasmic tail. An alternatively spliced isoform is truncated within the mucin-like stalk.TIM-3 is up‑regulated on several populations of activated myeloid cells (macrophage, monocyte, dendritic cell, microglia, mast cell) and T cells (Th1, CD8+, NK, Treg). Its binding to Galectin-9 induces a range of immunosuppressive functions which enhance immune tolerance and inhibit anti-tumor immunity . TIM-3 ligation attenuates CD8+ and Th1 cell responses and promotes the activity of Treg and myeloid derived suppressor cells. In addition, dendritic cell-expressed TIM-3 dampens inflammation by enabling the phagocytosis of apoptotic cells and the cross-presentation of apoptotic cell antigens. It also binds the alarmin HMGB1, thereby preventing the activation of TLRs in response to released tumor cell DNA. TIM-3 interactions with Galectin-9 can alternatively trigger immune stimulatory effects, such as the coactivation of NK cell cytotoxicity.

Product information

Synonym	HAVCR2; CD366; HAVcr-2; KIM-3; TIM3; TIMD-3; TIMD3; Tim-3; CD366; KIM-3; SPTCL; TIMD3; Tim-3; TIMD-3; HAVcr-2
Expression Host	HEK293 cells
Tag	C-hFc&His
Swiss-Prot
Gene ID

Usage guide

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.