Recombinant Human TNFRSF10D/DcR2/TRAIL-R4/CD264 Protein (RP00992)

Product name	Recombinant Human TNFRSF10D/DcR2/TRAIL-R4/CD264 Protein
Catalog No.	RP00992
Description	Active Recombinant Human TNFRSF10D/DcR2/TRAIL-R4 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala 56 - His 211 ) of human DcR2 (Accession #NP_003831.2) fused with an Fc, 6×His tag at the C-terminus.
Bio-Activity	1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human TRAIL at 2 μg/mL (100 μL/well) can bind Recombinant Human DcR2 with a linear range of 15-60 ng/mL. 2.Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L-929 mouse fibroblast cells treated with TRAIL. The ED₅₀ for this effect is 1.8-7.2 ng/mL in the presence of 20 ng/mL Recombinant Human TRAIL/TNFSF10.
Purity	> 95% by SDS-PAGE.
Endotoxin	< 0.1 EU/μg of the protein by LAL method.
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Species	Human
Calculated MW	43.59 kDa

Synonym	CD264; DCR2; TRAIL-R4; TRAILR4; TRUNDD; TNFRSF10D; DCR2; TRAIL-R4; TRAILR4; TRUNDD
Expression Host	HEK293 cells
Tag	C-hFc&His
Swiss-Prot
Gene ID

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.