| Product name | Recombinant Human TNFSF12/TWEAK Protein |
|---|---|
| Catalog No. | RP01446 |
| Description | Recombinant Human TNFSF12/TWEAK Protein is produced by E. coli expression system. The target protein is expressed with sequence (Lys97-His249（N-Met)) of human TWEAK/TNFSF12 (Accession #NP_003800.1) fused with no additional amino acid. |
| Bio-Activity | 1.Measured by its binding ability in a functional ELISA. Immobilized Human TNFSF12 at 2 μg/mL (100 μL/well) can bind Human TNFRSF12A with a linear range of 0.1-2.3 ng/mL. 2.Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 0.81-3.24 ng/mL. |
| Purity | > 95% by SDS-PAGE. |
| Endotoxin | <0.1EU/μg |
| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Species | Human |
| Calculated MW | 16.94 kDa |
| Synonym | TNFSF12; APO3L; DR3LG; TNLG4A; TWEAK |
|---|---|
| Expression Host | E. coli |
| Tag | No tag |
| Swiss-Prot | |
| Gene ID |
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
|---|---|
| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
* For research use only. Not for therapeutic or diagnostic purposes.
