| Product name | Recombinant Human TNFSF13B/BAFF/CD257 Protein |
|---|---|
| Catalog No. | RP00018 |
| Description | Recombinant Human TNFSF13B/BAFF/CD257 Protein is produced by E. coli expression system. The target protein is expressed with sequence ( Ala134-Leu285) of human TNFSF13B (Accession #NP_006564.1). |
| Bio-Activity | 1.Measured by its binding ability in a functional ELISA. Immobilized recombinant human BAFF at 5 μg/mL (100 μL/well) can bind recombinant human BCMA with a linear range of 3-20 ng/mL. 2.Measured in a cell proliferation assay using anti-IgM stimulated mouse B cells. The ED50 for this effect is 0.1-1 ng/mL, corresponding to a specific activity of 1×106-1×107units/mg. 3.Loaded Human TNFRSF17/BCMA/CD269 Protein, C-hFc&His (Catalog: RP00155) on ProA Biosensor, can bind Human TNFSF13B/BAFF/CD257 Protein, no Tag (Catalog: RP00018) with an affinity constant of 3.05nM as determined in BLI assay (Gator). |
| Purity | > 90% by SDS-PAGE. |
| Endotoxin | Please contact us for more information. |
| Formulation | Lyophilized from a 0.22 μm filtered solution of 50mM Tris, 150mM NaCl, pH 8.0.Contact us for customized product form or formulation. |
| Species | Human |
| Calculated MW | 17.04 kDa |
| Synonym | TNFSF13B; BAFF; BLYS; CD257; DTL; TALL-1; TALL1; THANK; TNFSF20; TNLG7A; ZTNF4 |
|---|---|
| Expression Host | E. coli |
| Tag | No tag |
| Swiss-Prot | |
| Gene ID |
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
|---|---|
| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
* For research use only. Not for therapeutic or diagnostic purposes.
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