| Product name | Recombinant Human TNFSF15/TL1 Protein |
|---|---|
| Catalog No. | RP00053 |
| Description | Recombinant Human TNFSF15/TL1 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Leu72-Leu251) of human TL1A/TNFSF15 (Accession #NP_005109.2) fused with an initial Met at the N-terminus. |
| Bio-Activity | 1.Measured by its binding ability in a functional ELISA. Immobilized Human TNFSF15 Protein at 5 μg/mL (100 μL/well) can bind DCR3 with a linear range of 0.12-6.98 ng/mL. 2.Measured by its ability to induce apoptosis of TF‑1 human erythroleukemic cells. The ED50 for this effect is 52.1-208.5 ng/mL, corresponding to a specific activity of 4.79×103~1.92×104 units/mg. |
| Purity | > 90% by SDS-PAGE. |
| Endotoxin | < 1.0 EU/μg of the protein by LAL method. |
| Formulation | Lyophilized from a 0.22 μm filtered solution of 20mM Tris, 50mM NaCl, 5% glycerol, pH 8.0.Contact us for customized product form or formulation. |
| Species | Human |
| Calculated MW | 20.47 kDa |
| Synonym | TNFSF15; TL1; TL1A; TNLG1B; VEGI; VEGI192A |
|---|---|
| Expression Host | E. coli |
| Tag | No tag |
| Swiss-Prot | |
| Gene ID |
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
|---|---|
| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
* For research use only. Not for therapeutic or diagnostic purposes.
