Overview
| Product name | Recombinant Human TNFSF6/FAS ligand/CD178 Protein |
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| Catalog No. | RP02353 |
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| Description | Recombinant Human TNFSF6/FAS ligand/CD178 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Pro134-Leu281) of Human Fas Ligand(monomer) fused with a His tag at the N-terminal. |
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| Bio-Activity | Measured by its ability to induce apoptosis of Jurkat human acute T cell leukemia cells. The ED50 for this effect is 2.075-8.3 ng/mL in the presence of 10 µg/mL of a cross-linking antibody Rabbit Anti-poly Histidine Monoclonal Antibody (Catalog # AE086), corresponding to a specific activity of 1.20×105~4.82×105 units/mg.
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| Purity | > 95% by Tris-Bis PAGE |
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| Endotoxin | < 1 EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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| Species | Human |
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| Calculated MW | 17.73 kDa |
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Product information
| Synonym | FASLG; ALPS1B; APT1LG1; APTL; CD178; CD95-L; CD95L; FASL; TNFSF6; TNLG1A; Fas ligand; FasL; FasLG |
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| Expression Host | HEK293 cells |
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| Tag | N-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
