Overview
| Product name | Recombinant Human TSHR Protein |
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| Catalog No. | RP03264 |
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| Description | Recombinant Human TSHR Protein is produced by Baculovirus-Insect Cells expression system. The target protein is expressed with sequence (Gly21-Gly413) of human TSHR (Accession #NP_000360.2) fused with His tag at the C-terminus. |
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| Purity | > 85% by SDS-PAGE. |
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| Endotoxin | < 1.0 EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of 50 mM Tris, 150 mM NaCl, 10% Glycerol, 1 mM TCEP, pH 7.5. Contact us for customized product form or formulation. |
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| Species | Human |
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| Calculated MW | 46.1 kDa |
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Background
Thyroid-stimulating hormone receptor (TSHR) is the receptor for the thyroid-stimulating hormone (TSH) or thyrotropin. TSH is secreted by the pituitary gland and promotes thyroid growth and function, with increased TSH levels typically associated with hypothyroidism. The activity of TSHR is mediated by G proteins which activate adenylate cyclase.
Product information
| Synonym | Thyrotropin receptor; Thyroid-stimulating hormone receptor; TSHR; TSH-R; LGR3; CHNG1; TSHR-I |
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| Expression Host | Baculovirus-Insect Cells |
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| Tag | C-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
