Recombinant Human Tissue-type plasminogen activator/TPA/PLAT Protein
Catalog No.
RP00595
Description
Recombinant Human Tissue-type plasminogen activator/TPA/PLAT Protein is produced by Human cells expression system. The target protein is expressed with sequence (Ser36-Pro562) of Human Tissue-type plasminogen activator/TPA/PLAT (Accession #P00750) fused with a 6×His tag at the C-terminus.
Purity
> 95% by SDS-PAGE.
Endotoxin
< 1 EU/μg of the protein by LAL method.
Formulation
Lyophilized from a 0.2 μm filtered solution of 20mM MES, 150mM NaCl, 0.2mM CaCl2, pH5.5. Contact us for customized product form or formulation.
Tissue-type plasminogen activator (PLAT) is a protein that secreted into extracellular space. PLAT contains five domains:EGF-like domain, fibronectin type-I domain, 2 kringle domains and peptidase S1 domain. It belongs to the peptidase S1family. The main function of this protein is to convert plasminogen into biologically active plasmin. As a protease, PLATplays a crucial role in regulating blood fibrinolysis, maintaining the homeostasis of extracellular matrix and in modulatingthe post-translational activation of growth factors. PLAT is found not only in the blood, where its primary function is as athrombolytic enzyme, but also in the central nervous system (CNS). It participates in a number of physiological andpathological events in the CNS, as well as the role of neuroserpin as the natural regulator of PLAT's activity in theseprocesses. Increased or decreased activity of PLAT leads to hyperfibrinolysis or hypofibrinolysis, respectively. In addition, asa cytokine, PLAT plays a pivotal role in the pathogenesis of renal interstitial fibrosis through diverse mechanisms. Thus, as afibrogenic cytokine, it promotes the progression of kidney diseases.
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
* For research use only. Not for therapeutic or diagnostic purposes.
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