Overview
| Product name | Recombinant Human Transcriptional Repressor Ctcf/CTCF Protein |
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| Catalog No. | RP00606 |
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| Description | Recombinant Human Transcriptional Repressor Ctcf/CTCF Protein is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Ile154) of Human Transcriptional Repressor Ctcf/CTCF (Accession #P49711)fused with no tag.. |
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| Purity | > 95% by SDS-PAGE. |
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| Endotoxin | < 1 EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.2 μM filtered solution of PBS, pH 7.4Contact us for customized product form or formulation. |
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| Species | Human |
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| Calculated MW | 16.9 kDa |
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Background
Transcriptional Repressor CTCF (CTCF) belongs to the CTCF Zinc-Finger Protein family. CTCF contains twelveC2H2-type zinc fingers and interacts with CHD8. CTCF is widely expressed in many tissues, and it is absent inprimary spermatocytes. CTCF is involved in transcriptional regulation by binding to chromatin insulators andpreventing interaction between promoter and nearby enhancers and silencers. CTCF plays an essential role inoocyte and preimplantation embryo development by activating or repressing transcription. In addition, CTCF isalso indispensable in the epigenetic regulation and chromatin remodeling.
Product information
| Synonym | CTCF; MRD21; CCCTC-binding factor; MRD21; CTCF |
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| Expression Host | E. coli |
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| Tag | No tag |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
