Overview
| Product name | Recombinant Human Wnt-3a Surrogate Protein |
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| Catalog No. | RP01618 |
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| Description | Recombinant Human Wnt-3a Protein is produced by HEK293 cells expression system. The target protein is fused with a hFc tag at the C-terminus. |
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| Bio-Activity | Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 3.95-15.79 ng/mL, corresponding to a specific activity of 6.33×104~2.53×105 units/mg.
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| Purity | > 95% by SDS-PAGE. |
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| Endotoxin | <1EU/μg |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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| Species | Human |
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| Calculated MW | 57.12 kDa |
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Product information
| Synonym | MGC119418; MGC119419; MGC119420; protein Wnt-3a; wingless-type MMTV integration site family; member 3A; Wnt3a; Wnt-3a |
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| Expression Host | HEK293 cells |
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| Tag | C-hFC |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
