Overview
| Product name | Recombinant Human beta Defensin 3/DEFB103A Protein |
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| Catalog No. | RP02867 |
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| Description | Recombinant Human beta Defensin 3/DEFB103A Protein is produced by E. coli expression system. The target protein is expressed with sequence (Gly23-Lys67) of human beta Defensin 3/DEFB103A (Accession #NP_061131.1) fused with a 6×His tag at the N-terminus. |
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| Purity | >95% as determined by SDS-PAGE |
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| Endotoxin | Please contact us for more information. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of 50mM Tris, 0.3% Ttiton X-100, 0.3% SKL, pH 8.5. |
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| Species | Human |
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| Calculated MW | 7.3 kDa |
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Background
Beta-defensin 3 is a member of the defensin family. Defensin family is comprised by microbicidal and cytotoxic peptides made by neutrophils. Members of the beta-defensin 3 family are highly similar in protein sequence. Beta-defensin 3 shows antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Beta-defensin 3 is abundantly expressed in skin and tonsils, and to a lesser extent in trachea, uterus, kidney, thymus, adenoid, pharynx and tongue. It is also expressed in salivary gland, bone marrow, colon, stomach, polyp and larynx. However, in small intestine, it cannot be detected. Defensin has broad spectrum antimicrobial activity and may play an important role in innate epithelial defense. Beta-defensin 3 kills multiresistant S.aureus and vancomycin-resistent E.faecium. It has no significant hemolytic activity.
Product information
| Synonym | Beta-defensin 3; BD-3; DEFB-3; HBD3; hBD-3; DEFB103A |
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| Expression Host | E. coli |
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| Tag | N-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
