Overview
| Product name | Recombinant Monkeypox virus M1R Protein |
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| Catalog No. | RP03292 |
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| Description | Recombinant Monkeypox virus M1R Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly2-Gly183) of Monkeypox virus M1R (Accession #QJQ40223.1) fused with His tag at the C-terminus. |
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| Purity | > 95% by Tris-Bis PAGE and HPLC. |
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| Endotoxin | < 1.0 EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation. |
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| Species | Monkeypox virus |
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| Calculated MW | 20.41 kDa |
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Background
Monkeypox virus (MPXV) is double-stranded DNA virus belonging to the genus orthopoxvirus that causes a smallpox-like disease in humans. M1R is homologous to the vaccinia virus L1 protein, a transmembrane protein found on the surface of mature IMV particles. And M1R is the component of the entry fusion complex (EFC), which consists of 11 proteins. During cell infection, this complex mediates entry of the virion core into the host cytoplasm by a two-step mechanism consisting of lipid mixing of the viral and cellular membranes and subsequent pore formation.
Product information
| Synonym | M1R; Monkeypox virus M1R; MPXV M1R |
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| Expression Host | HEK293 cells |
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| Tag | C-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃. Store the lyophilized protein at -20℃ to -80℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
