Overview
| Product name | Recombinant Mouse ALK-4/ACVR1B Protein |
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| Catalog No. | RP01803 |
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| Description | Recombinant Mouse ALK-4/ACVR1B Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Glu126) of mouse ALK-4/ACVR1B (Accession #NP_031421.1) fused with hFC tag at the C-terminus. |
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| Bio-Activity | Measured by its binding ability in a functional ELISA. Immobilized Mouse TDGF1 (Catalog: RP01946) at 5 μg/mL (100 μL/well) can bind Mouse ALK4(Catalog: RP01803) with a linear range of 4.5-133.2 ng/mL.
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| Purity | > 92% by SDS-PAGE. |
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| Endotoxin | < 0.01EU/μg |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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| Species | Mouse |
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| Calculated MW | 37.30 kDa |
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Background
ACVR1B (Activin A Receptor, type 1B), belongs to the protein kinase superfamily, TKL Ser/Thr protein kinase family, and TGFB receptor subfamily. ALK-4/ACVR1B acts as a transducer of activin or activin like ligands signals. Activin binds to either ACVR2A or ACVR2B and then forms a complex with ACVR1B. The known type II activin receptors include ActRII and ActRIIB, while the main type I activin receptor in mammalian cells is ALK-4 (ActRIB). In the presence of activin, type II and type I receptors form complexes whereby the type II receptors activate ALK-4 through phosphorylation. The activated ALK-4, in turn, transduces signals downstream by phosphorylation of its effectors, such as Smads, to regulate gene expression and affect cellular phenotype. ALK-4/ACVR1B is an important regulator of vertebrate development, with roles in mesoderm induction, primitive streak formation, gastrulation, dorsoanterior patterning, and left-right axis determination.
Product information
| Synonym | Alk4; SKR2; ALK-4; ActRIB; ActR-IB; Acvrlk4; 6820432J04 |
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| Expression Host | HEK293 cells |
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| Tag | C-hFc |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
