Overview
| Product name | Recombinant Mouse Apolipoprotein E/APOE Protein |
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| Catalog No. | RP00640 |
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| Description | Recombinant Mouse ApoE/APOE/Apolipoprotein E Protein is produced by Human cells expression system. The target protein is expressed with sequence (Glu19-Gln311) of mouse ApoE/APOE/Apolipoprotein E (Accession #P08226) fused with a 6×His tag at the C-terminus. |
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| Bio-Activity | Measured by its binding ability in a functional ELISA. Immobilized Mouse APOE(Catalog: RP00640 ) at 5μg/mL (100 μL/well) can bind Mouse TREM2(Catalog: RP01558) with a linear range of 0.16-1.5μg/mL.
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| Purity | > 95% by SDS-PAGE. |
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| Endotoxin | < 1 EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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| Species | Mouse |
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| Calculated MW | 34.81 kDa |
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Background
Apolipoprotein E (Apo-E), is a member of the apolipoprotein A1/A4/E family. ApoE is a major proteincomponent of serum LDL, VLDL, HDL, and chylomicrons. APOE may function in mediating the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptorand for the specific apo-E receptor (chylomicron remnant) of hepatic tissues. APOE is usually secreted inplasma. Phosphorylation sites are present in the extracellular medium.
Product information
| Synonym | APOE; AD2; APO-E; ApoE4; LDLCQ5; LPG; AI255918; Apo-E; Apoe |
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| Expression Host | HEK293 cells |
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| Tag | C-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
