Overview
| Product name | Recombinant Mouse Autotaxin/E-NPP2 Protein |
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| Catalog No. | RP01852 |
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| Description | Recombinant Mouse Autotaxin/E-NPP2 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ser49-Ile862) of Mouse Autotaxin/E-NPP2 (Accession #NP_056559.2) fused with a His tag at the C-terminus. |
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| Endotoxin | <0.1EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of 20mM Tris，150mM NaCl，pH7.4 |
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| Species | Mouse |
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| Calculated MW | 94.40 kDa |
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Background
ENPP-2, also known as Autotaxin, belongs to the ectonucleotide pyrophosphatase/phosphodiesterase (NPP) family. Some NPPs hydrolyze phosphates from nucleotides and their derivatives. ENPP-2 shares 40 - 50% identity to ENPP1 & 3, all of which contain a N-terminal intracellular domain, a single transmembrane domain and a large extracellular domain that includes a catalytic domain, two somatomedin-B-like domains, and a C-terminal nuclease-like domain . Unlike ENPP-1 and ENPP-3, ENPP-2 has weak activity against nucleotides, but exhibits a lysophospholipase D activity which allows the formation of lysophosphatidic acid (LPA) and choline from lysophosphatidylcholine . The hydrolysis of nucleotides and lysophospholipids by ENPP-2 is mediated by a single catalytic site . Evidence shows LPA and sphingosine 1-phosphate to be specific inhibitors of ENPP-2 . ENPP-2 was originally found to stimulate tumor cell motility and has since been found to enhance tumor invasion and metastasis ( and to be up-regulated in several types of carcinomas including breast and lung .
Product information
| Synonym | Ectonucleotide pyrophosphatase/phosphodiesterase family member 2; E-NPP 2; 3.1.4.39; Autotaxin; Extracellular lysophospholipase D; LysoPLD，Enpp2; Npps2; Pdnp2 |
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| Expression Host | HEK293 cells |
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| Tag | C-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
