Overview
| Product name | Recombinant Mouse CCL21/6Ckine Protein |
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| Catalog No. | RP01885 |
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| Description | Recombinant Mouse CCL21A Protein is produced by Pichia expression system. The target protein is expressed with sequence (Ser24-Gly133) of Mouse CCL21A (Accession #P84444) fused with a His tag at the C-terminus. |
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| Endotoxin | < 0.1 EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.Contact us for customized product form or formulation. |
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| Species | Mouse |
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| Calculated MW | 12.89 kDa |
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Background
C-C Motif Chemokine 21a (CCL21a) is a small secreted cytokine that belongs to the intercrine beta (CCchemokine) family. Mouse CCL21 cDNA encodes a 133 amino acid residue protein with a 23 residue signalpeptide that is cleaved to generate the 110 residue mature protein. Mouse CCL21 has three forms whileCCL21a has Ser-65. CCL21 elicits its effects by binding to a cell surface chemokine receptor known as CCR7 andCXCR3. Mouse CCL21 inhibits hemopoiesis and stimulates chemotaxis. It has chemotactic function in vitro forthymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Mouse CCL21 showspreferential activity towards naive T-cells and may play a role in mediating homing of lymphocytes tosecondary lymphoid organs.
Product information
| Synonym | C-C Motif Chemokine 21a; 6Ckine; Beta-Chemokine Exodus-2; Small-Inducible Cytokine A21a; Thymus-Derived Chemotactic Agent 4; TCA4; Ccl21a; Scya21; Scya21a |
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| Expression Host | Pichia |
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| Tag | C-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
