Overview
| Product name | Recombinant Mouse CD28 Protein |
|---|
| Catalog No. | RP01211 |
|---|
| Description | Recombinant Mouse CD28 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asn20-Lys149) of mouse CD28 (Accession #NP_031668.3) fused with a Fc, 6×His tag at the C-terminus. |
|---|
| Bio-Activity | 1.Measured by its binding ability in a functional ELISA. Immobilized Mouse CD86 Protein at 5 μg/mL (100 μL/well) can bind Mouse CD28 with a linear range of 0.0012-1.154 μg/mL.
|
|---|
| Purity | > 97% by SDS-PAGE. |
|---|
| Endotoxin | < 0.1 EU/μg of the protein by LAL method. |
|---|
| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
|---|
| Species | Mouse |
|---|
| Calculated MW | 41.84 kDa |
|---|
Product information
| Synonym | CD28; Tp44; CD28 |
|---|
| Expression Host | HEK293 cells |
|---|
| Tag | C-hFc&His |
|---|
| Swiss-Prot | |
|---|
| Gene ID | |
|---|
Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
|---|
| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
|---|
* For research use only. Not for therapeutic or diagnostic purposes.
i_FC_01.jpg?t=1702609105)
Y_FC_01.jpg?t=1711069530)
