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Recombinant Mouse Calcitonin/CALCA Protein (RP02196)

Publication (1) Datasheet

Overview

Product nameRecombinant Mouse Calcitonin/CALCA Protein
Catalog No.RP02196
DescriptionRecombinant Mouse Calcitonin/CALCA Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Val26-Asp119) of mouse  Calcitonin/CALCA (Accession #NP_001029126.1) fused with  hFc tag at the C-terminus.
Purity> 95% as determined by HPLC.
Endotoxin<1EU/μg
FormulationLyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
SpeciesMouse
Calculated MW36.9 kDa
Specific fragments of methylation changes in the target gene (Calca) were identified by IGV analysis. CGRP was applied to compare the effects on ASCs-T2DM morphology via phalloidin staining, proliferation through CCK-8 assay, and osteogenic differentiation with osteogenic staining, qPCR, and repair of calvarial defect. Furthermore, 5-azacytidine (5-az) was used to intervene ASCs-T2DM to verify the relationship between the methylation level of the target fragment and expression of Calca.
SynonymCalcitonin; CCP; PDN-21; CALCA; CALC1; CGRP; CGRP-I; CGRP1; CT; KC; PCT; Procalcitonin; Calcitonin 1; Katacalcin
Expression HostHEK293 Cells
TagC-hFc
Swiss-Prot
Gene ID
StorageStore at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
ReconstitutionCentrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.

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