Overview
| Product name | Recombinant Mouse Cathepsin B Protein |
|---|
| Catalog No. | RP02994 |
|---|
| Description | Recombinant Mouse Cathepsin B Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (His18-Phe339) of mouse Cathepsin B (Accession #NP_031824.1) fused with a 6×His tag at the C-terminus. |
|---|
| Bio-Activity | Measured by its ability to cleave the fluorogenic peptide substrate Z-LR-AMC (R&D Systems, Catalog # ES008). The specific activity is >2, 000 pmoles/min/μg.
|
|---|
| Purity | >95% as determined by SDS-PAGE |
|---|
| Endotoxin | <1EU/μg |
|---|
| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
|---|
| Species | Mouse |
|---|
| Calculated MW | 36.6 kDa |
|---|
Product information
| Synonym | Cathepsin B; 3.4.22.1; APP secretase; APPS; Cathepsin B1; CTSB |
|---|
| Expression Host | HEK293 cells |
|---|
| Tag | C-His |
|---|
| Swiss-Prot | |
|---|
| Gene ID | |
|---|
Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
|---|
| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
|---|
* For research use only. Not for therapeutic or diagnostic purposes.
