Overview
| Product name | Recombinant Mouse E-Cadherin/CDH1/CD324 Protein |
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| Catalog No. | RP01727 |
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| Description | Recombinant Mouse E-Cadherin/CDH1/CD324 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asp157-Val709) of mouse E-Cadherin/Cadherin-1/CDH1 (Accession #NP_033994.1) fused with and a 6×His tag at the C-terminus. |
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| Bio-Activity | Measured by the ability of the immobilized protein to support the adhesion of the MCF-7 human breast cancer cells. The ED50 for this effect is 0.82-3.29 μg/mL.
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| Endotoxin | < 0.1EU/μg |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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| Species | Mouse |
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| Calculated MW | 61.70 kDa |
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Background
This protein is a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer. Alternate transcriptional splice variants of this gene have been found, but only three have been characterized to date.
Product information
| Synonym | Um; UVO; Ecad; ARC-1; E-cad; L-CAM; CDH1 |
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| Expression Host | HEK293 cells |
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| Tag | C-6His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
