제품 > 재조합 단백질

Recombinant Mouse EGF Protein (RP01684)

Bioactivityvalidated

Datasheet

Overview

Product nameRecombinant Mouse EGF Protein
Catalog No.RP01684
DescriptionRecombinant Mouse EGF Protein is produced by Pichia expression system. The target protein is expressed with sequence (Asn977-Arg1029) of mouse EGF (Accession #NP_034243.2) fused with no additional amino acid.
Bio-ActivityMeasured in a cell proliferation assay using BALB/3T3 mouse fibroblasts. The ED50 for this effect is 37-148 pg/mL, corresponding to a specific activity of 7.14×106~2.50×107 units/mg.
Purity> 97% by SDS-PAGE.
Endotoxin<1EU/μg
FormulationLyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
SpeciesMouse
Calculated MW6.45 kDa
EGF is the founding member of the EGF-family of proteins. Members of this protein family have highly similar structural and functional characteristics. EGF contains 9 EGF-like domains and 9 LDL-receptor class B repeats. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bonds. As a low-molecular-weight polypeptide, EGF was first purified from the mouse submandibular gland, but since then it was found in many human tissues including submandibular gland, parotid gland.
Synonymbeta-urogastrone; EGF; epidermal growth factor (beta-urogastrone); epidermal growth factor; HOMG4; pro-epidermal growth factor; URG; Urogastrone
Expression HostPichia
TagNo-tag
Swiss-Prot
Gene ID
StorageStore at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
ReconstitutionCentrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.

용량을 선택하세요

16 Sep 2024-20 Dec 2024 on sale

문의하기