Overview
| Product name | Recombinant Mouse EGFR/HER1 Protein |
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| Catalog No. | RP03240 |
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| Description | Recombinant Mouse EGFR/HER1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu25-Ser647) of Mouse EGFR/HER1 (Accession #NP_997538.1) fused with a His tag at the C-terminus. |
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| Bio-Activity | Recombinant Mouse EGFR/HER1 Protein captured on CM5 Chip via anti-his antibody can bind Mouse EGF, hFc Tag with an affinity constant of 9.96 nM as determined in SPR assay (Biacore T200).
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| Purity | > 95% by Tris-Bis PAGE and HPLC. |
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| Endotoxin | <1EU/ug of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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| Species | Mouse |
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| Calculated MW | 70.60 kDa |
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Background
As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.
Product information
| Synonym | Egfr; Epidermal growth factor receptor |
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| Expression Host | HEK293 cells |
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| Tag | C-6His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
