Overview
| Product name | Recombinant Mouse Ephrin-B3/EFNB3 Protein |
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| Catalog No. | RP01926 |
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| Description | Recombinant Recombinant Mouse Ephrin-B3/EFNB3 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu28-Ala227) of Mouse Ephrin-B3/EFNB3 (Accession #NP_031937.1) fused with hFc tag at the C-terminus. |
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| Bio-Activity | Measured by its binding ability in a functional ELISA. Immobilized Mouse EphB2(Catalog: RP01156) at 2 μg/mL (100 μL/well) can bind Mouse Efnb3 (Catalog: RP01926) with a linear range of 0.50-58.76 ng/mL.
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| Purity | > 95% by SDS-PAGE. |
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| Endotoxin | <0.01EU/μg of the protein by LAL method |
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| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
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| Species | Mouse |
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| Calculated MW | 47.88 kDa |
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Background
Ephrin B3 belongs to the ephrin family. Ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. Ephrin B3 is important in brain development as well as in its maintenance. It is especially important for forebrain function since its expression levels were particularly high in several forebrain subregions compared to other brain subregions. Ephrin B3 binds to, and induce the collapse of, commissural axons/growth cones in vitro.
Product information
| Synonym | Ephrin-B3; Efnb3 |
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| Expression Host | HEK293 cells |
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| Tag | C-hFC |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
