Overview
| Product name | Recombinant Mouse FGF-10 Protein |
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| Catalog No. | RP01848 |
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| Description | Recombinant Mouse FGF-10 Protein is produced by E. coli expression systerm. The target protein is expressed with sequence (Gln37-Thr209) of Mouse FGF-10 (Accession #NP_032028.1) fused with no tag. |
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| Bio-Activity | Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. The ED50 for this effect is 3.66-14.64 ng/mL, corresponding to a specific activity of 56.83×104~2.73×105 units/mg.
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| Endotoxin | <1EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of 20mMPB, 800mMNaCl，pH7.4 |
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| Species | Mouse |
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| Calculated MW | 19.54 kDa |
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Background
Fibroblast Growth Factor 10 (FGF 10) is an evolutionary conserved secreted growth factor mediating mostly mesenchymal to epithelial signaling. FGF 10 belongs to the FGF 7 subfamily and shares similar biochemical and amino acid sequences with its constituent members (FGF3, FGF 7 and FGF 22). As a paracrine FGF, FGF 10 elicits its biological responses by activating the fibroblast growth factor receptor 2b (FGF R 2b), is crucial for governing proximal distal outgrowth as well as patterning and acts upstream of the known apical ectodermal ridge (AER) marker FGF 8. FGF10 is also implicated in pancreatic cancer, and that overexpression of FGFR2b is associated with metastatic invasion.
Product information
| Synonym | Fibroblast growth factor 10; FGF-10; Keratinocyte growth factor 2; Fgf10 |
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| Expression Host | E. coli |
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| Tag | NO-Tag |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
