Overview
| Product name | Recombinant Mouse FGF-6 Protein |
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| Catalog No. | RP01860 |
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| Description | Recombinant Mouse FGF-6 Protein is produced by E.coli expression system. The target protein is expressed with sequence (Ser38-Ile208) of Mouse FGF-6 (Accession #NP_034334.1) fused with No-Tag. |
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| Bio-Activity | Measured in a cell proliferation assay using Balb3T3 mouse fibroblast cells. The ED50 for this effect is 0.52-2.06 ng/mL, corresponding to a specific activity of 4.85×105~1.92×106 units/mg.
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| Endotoxin | <1EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 800 mM NaCl, pH7.4. |
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| Species | Mouse |
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| Calculated MW | 18.93 kDa |
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Background
FGF-6 is a member of the large and diverse fibroblast growth factor (FGF) family of peptide growth factors. FGF-6 binds to receptor FGFR4 and functions as a signaling molecule that is involved in many important processes . The mouse homolog of this gene exhibits predominantly in the myogenic lineage which suggests a role in muscle regeneration or differentiation . FGF-6 was able to transform NIH/3T3 cells and the resulting transformant formed a well-vascularized tumor in nude mice, thus suggesting an angiogenic property similar to some other members of the FGF family .
Product information
| Synonym | Fgf6; Fgf-6; Hstf2; Fibroblast growth factor 6; FGF-6; Heparin secretory-transforming protein 2; HST-2; HSTF-2; Heparin-binding growth factor 6; HBGF-6. |
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| Expression Host | E.coli |
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| Tag | No-Tag |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
