Overview
| Product name | Recombinant Mouse FGF-9 Protein |
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| Catalog No. | RP01840LQ |
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| Description | Recombinant Mouse FGF-9 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Ser208) of Mouse FGF-9 (Accession #NP_038546.2) fused with NO-tag. |
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| Bio-Activity | Measured in a cell proliferation assay using BALB/3T3 mouse fibroblasts. The ED50 for this effect is 2.52-10.06 ng/mL, corresponding to a specific activity of 9.94×104~3.97×105 units/mg.
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| Purity | > 90% by SDS-PAGE. |
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| Endotoxin | < 0.1 EU/μg of the protein by LAL method. |
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| Formulation | Supplied as a 0.22 μm filtered solution of solution of 20mM Tris-HCl, 250mM NaCl, 1mM EDTA, 20% Glycerol, 1mM DTT, pH 8.5 |
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| Species | Mouse |
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| Calculated MW | 23.41 kDa |
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Background
Fibroblast growth factor 9 is also known as FGF9, GAF, HBFG-9, SYNS3, and is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF9 is also a mitogen for oligodendrocyte type 2 astrocyte progenitor cells, smooth muscle cells, pheochromocytoma PC12 cells, and BALB/3T3 fibroblasts. However, unlike FGF acidic and basic, FGF9 has no effect on human umbilical vein endothelial cells, and it has been demonstrated that FGF9 binds preferentially to the IIIc form of FGFR3. Although no typical signal sequence was found in FGF9, it is secreted efficiently after synthesis not in a conventional manner. In nervous system, FGF9 is produced mainly by neurons and may plays an important role in CNS development. FGF9 has been shown to interact with Fibroblast growth factor receptor 3.
Product information
| Synonym | Fibroblast growth factor 9; FGF-9; Glia-activating factor; GAF; HBGF-9，Fgf9; Fgf-9 |
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| Expression Host | E. coli |
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| Tag | No-tag |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -70℃. This product is stable at ≤ -70℃ for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles. |
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| Reconstitution | Centrifμge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
