| Product name | Recombinant Mouse FSTL3 Protein |
|---|---|
| Catalog No. | RP03254 |
| Description | Recombinant Mouse FSTL3 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val24-Val256) of Mouse FSTL3 (Accession #Q9EQC7) fused with His tag at the C-terminus. |
| Bio-Activity | 1. Immobilized Human Activin A, No Tag at 0.2 μg/mL (100 μL/well) on the plate. Dose response curve for Mouse FSTL3, His Tag with the EC50 of 18.0 ng/mL determined by ELISA. 2. Mouse FSTL3, His Tag captured on CM5 Chip via anti-his antibody can bind Human Activin A, No tag with an affinity constant of 53.09 pM as determined in SPR assay (Biacore T200). 3. Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 5-25 ng/mL in the presence of 7.5 ng/mL rhActivin A. |
| Purity | > 95% by Tris-Bis PAGE and HPLC. |
| Endotoxin | < 1.0 EU/μg of the protein by LAL method. |
| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation. |
| Species | Mouse |
| Calculated MW | 26 kDa |
| Synonym | Follistatin-like protein 3; FLRG; Follistatin-like 3; FSTL3 |
|---|---|
| Expression Host | HEK293 cells |
| Tag | C-His |
| Swiss-Prot | |
| Gene ID |
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
|---|---|
| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
* For research use only. Not for therapeutic or diagnostic purposes.
