| Product name | Recombinant Mouse GAS-6 Protein |
|---|---|
| Catalog No. | RP02814 |
| Description | Recombinant Mouse GAS-6 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val28-Pro674) of mouse GAS-6 (Accession #NP_062394.2) fused with a 6×His tag at the C-terminus. |
| Bio-Activity | 1.Immobilized Mouse GAS6, His Tag at 5μg/m l (100μl/well) on the plate. Dose response curve for Human AXL, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA (QC Test). 2.Mouse AXL, His Tag immobilized on CM5 Chip can bind Mouse GAS6, His Tag with an affinity constant of 15.62 nM as determined in SPR assay (Biacore T200) (Routine Test). |
| Purity | > 95% as determined by HPLC |
| Endotoxin | <1EU/μg |
| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Species | Mouse |
| Calculated MW | 73.09 kDa |
| Synonym | GAS6; AXLLG; AXSF |
|---|---|
| Expression Host | HEK293 cells |
| Tag | C-His |
| Swiss-Prot | |
| Gene ID |
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
|---|---|
| Reconstitution | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
* For research use only. Not for therapeutic or diagnostic purposes.
