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Recombinant Mouse IFN-alpha 2 Protein (RP01725)

Bioactivityvalidated

Publication (2) Datasheet

Overview

Product nameRecombinant Mouse IFN-alpha 2 Protein
Catalog No.RP01725
DescriptionRecombinant Mouse IFN-alpha 2 Protein is produced by Pichia expression system. The target protein is expressed with sequence (Cys24-Glu190) of mouse IFN-alpha 2 (Accession #) fused with no additional amino acid.
Bio-ActivityMeasured in a cell cytotoxicity assay using TF-1 cells. The ED50 for this effect is 51.80‑207.20 ng/mL, corresponding to a specific activity of 4.83×103~1.93×104 units/mg.
Endotoxin< 0.1EU/μg
FormulationLyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
SpeciesMouse
Calculated MW19.33 kDa
IFNA2 (Interferon Alpha 2) is a Protein Coding gene. This gene is a member of the alpha interferon gene cluster on chromosome 9. The encoded protein is a cytokine produced in response to viral infection. Type I Interferons (IFNs) are well-known cytokines that exert antiviral activity, antitumor activity, and immunomodulatory effects. Interferon tau (IFNT), a type I IFN similar to alpha IFNs (IFNA), is the pregnancy recognition signal produced by the ruminant conceptus. Among the IFN-α genes, a total of 28 different sequence variants have been described. The three principal subtypes of IFNα-2 are designated α-2a, α-2b, and α-2c. IFNα-2b is being the predominant allele while IFNα-2a is less predominant and IFNα-2c only a minor allelic variant.
SynonymIFNA2; IFN-alphaA; IFNA; IFNA2B; INFA2; interferon alpha-2; Interferon alpha 2 (IFN-α2); IFN-alphaA; IFNA; IFNA2B; INFA2
Expression HostPichia
TagNO-tag
Swiss-Prot
Gene ID
StorageStore at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
ReconstitutionCentrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.

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