Overview
| Product name | Recombinant Mouse IL-36 gamma/IL-1F9 Protein |
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| Catalog No. | RP01762 |
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| Description | Recombinant Mouse IL-36 gamma/IL-1F9 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly13-Ser164) of mouse IL36G (Accession #) fused with and a 6×His tag at the C-terminus. |
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| Bio-Activity | Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677. The ED 50 for this effect is 0.1-0.4 μg/mL, corresponding to a specific activity of 2.5×103~1×104 units/mg.
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| Purity | > 95% by SDS-PAGE. |
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| Endotoxin | < 0.01EU/μg |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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| Species | Mouse |
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| Calculated MW | 18.17 kDa |
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Background
The protein is a member of the interleukin 1 cytokine family. The activity of this cytokine is mediated by interleukin 1 receptor-like 2 (IL1RL2/IL1R-rp2), and is specifically inhibited by interleukin 1 family, member 5 (IL1F5/IL-1 delta). Interferon-gamma, tumor necrosis factor-alpha and interleukin 1, beta (IL1B) are reported to stimulate the expression of this cytokine in keratinocytes. The expression of this cytokine in keratinocytes can also be induced by a contact hypersensitivity reaction or herpes simplex virus infection.
Product information
| Synonym | If36g; Il1f9; IL-36gamma |
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| Expression Host | HEK293 cells |
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| Tag | C-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose). |
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* For research use only. Not for therapeutic or diagnostic purposes.
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