Recombinant Mouse IL-4R alpha/CD124 Protein (RP01680)

Overview

Product name	Recombinant Mouse IL-4R alpha/CD124 Protein
Catalog No.	RP01680
Description	Recombinant Mouse IL-4R alpha/CD124 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ile 26 - Arg 233) of mouse IL4R (Accession #NP_001008700.1) fused with and a 6×His tag at the C-terminus.
Bio-Activity	Measured by its ability to inhibit the mIL-4-dependent proliferation of HT-2 mouse T cells. The ED₅₀ for this effect is 0.5-2 μg/mL in the presence of 5 ng/mL of recombinant mouse IL-4(Catalog: RP01161).
Purity	> 97% by SDS-PAGE.
Endotoxin	<0.1EU/μg
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Species	Mouse
Calculated MW	25.22 kDa

CD124, also known as the interleukin 4 receptor (IL4R), is a typeⅠ transmembrane protein that can regulate IgE antibody production in B cells through binding to interleukin 4 and interleukin 13 and promote differentiation of Th2 cells through binding to interleukin 4. The membrane-bound form of CD124 can be hydrolyzed to a soluble form which can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells.

Product information

Synonym	Il4r; CD124；IL-4R
Expression Host	HEK293 cells
Tag	C-6His
Swiss-Prot
Gene ID

Usage guide

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.