Recombinant Mouse IL-6 Protein (RP01321)

Overview

Product name	Recombinant Mouse IL-6 Protein
Catalog No.	RP01321
Description	Recombinant Mouse IL-6 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Phe25-Thr211) of Mouse IL6 (Accession #NP_112445.1) fused with an 6×His tag at the C-terminus.
Bio-Activity	Measured in a cell proliferation assay using NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED₅₀ for this effect is 0.07-0.28 ng/mL, corresponding to a specific activity of 3.57×10⁶ ~ 1.43×10⁷ units/mg.
Purity	> 95% by SDS-PAGE.
Endotoxin	< 0.1 EU/μg of the protein by LAL method.
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Species	Mouse
Calculated MW	22.57 kDa

Interleukin-6 is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions.It is secreted by T cells, macrophages , monocytes, fibroblasts, endothelial cells, et.al. to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation.Interleukin 6 has been shown to interact with interleukin-6 receptor and glycoprotein. IL-6 is relevant to many disease processes such as diabetes, atherosclerosis, depression, Alzheimer's Disease, systemic, lupus erythematosus, prostate cancer and rheumatoid arthritis.

Product information

Synonym	IL6; Interleukin-6; BSF2; HSF; IFNB2; IL6
Expression Host	HEK293 cells
Tag	C-His
Swiss-Prot
Gene ID

Usage guide

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.