Overview
| Product name | Recombinant Mouse IgE Protein |
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| Catalog No. | RPT0013 |
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| Description | Recombinant Mouse IgE Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Pro50-Lys388) of mouse IgE (Accession #) fused with and 6×His tag at the C-terminus. |
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| Bio-Activity | Measured by its binding ability in a functional ELISA. Immobilized Mouse IgE (Catalog: RPT0013) at 1 μg/mL (100 μL/well) can bind Mouse IgE Rabbit mAb(Catalog: A23195) with a linear range of 0.06-0.45 ng/mL.
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| Endotoxin | < 0.1 EU/μg |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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| Species | Mouse |
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| Calculated MW | 38.99 kDa |
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Background
As one of the five designated immunoglobulin isotypes, immunoglobulin E (IgE) plays a major role in atopic conditions by inducing immediate hypersensitivity reactions. IgE also contributes significantly to the body’s immune response to parasitic infections. IgE antibodies are predominantly found in the tissues, firmly attached to effector cells, such as mast cells and basophils, by high-affinity IgE Fc receptor (Fc epsilon RI) and low-affinity IgE receptor (Fc epsilon RII).
Product information
| Synonym | IgE |
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| Expression Host | HEK293 cells |
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| Tag | C-His |
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| Swiss-Prot | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
