Overview
| Product name | Recombinant Mouse LAG-3/CD223 Protein |
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| Catalog No. | RP01327 |
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| Description | Recombinant Mouse LAG-3/CD223 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly24-Leu442) of Mouse LAG3 (Accession #NP_032505.1) fused with an 6×His tag at the C-terminus. |
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| Bio-Activity | Measured by its binding ability in a functional ELISA.Immobilized Mouse LAG3 (Catalog: RP01327) at 1 μg/mL (100 μL/well) can bind LAG3 Rabbit pAb with a linear range of 0.06-1.2ng/mL.
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| Purity | > 95% by SDS-PAGE. |
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| Endotoxin | < 0.1 EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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| Species | Mouse |
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| Calculated MW | 46.18 kDa |
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Background
LAG3 (Lymphocyte Activating 3), also known as CD223 and Lymphocyte activation gene 3, belongs to the immunoglobulin (Ig) superfamily, is a protein-coding gene. 2 alternatively spliced human isoforms have been reported. The LAG3 gene contains 8 exons. It is selectively expressed in activated T and NK cells. LAG3 contains 4 extracellular Ig-like domains and has a negative regulatory function in T cells. It also acts as a new marker of T cell-induced B cell activation. As a soluble molecule, LAG3 activates antigen-presenting cells through MHC class II signaling, leading to increased antigen-specific T-cell responses in vivo. Diseases associated with LAG3 include Smoldering Myeloma and Kyphoscoliotic Heart Disease.
Product information
| Synonym | LAG3; CD223; FDC; Ly66; LAG3 |
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| Expression Host | HEK293 cells |
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| Tag | C-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
