Overview
Product name | Recombinant Mouse LEU3/CD4 Protein |
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Catalog No. | RP02947 |
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Description | Recombinant Mouse LEU3/CD4 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Lys27-Thr394) of mouse CD4 (Accession #NP_038516.1) fused with a 6×His tag at the C-terminus. |
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Purity | > 95% as determined by HPLC |
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Endotoxin | <1EU/μg |
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Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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Species | Mouse |
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Calculated MW | 42.4 kDa |
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Background
CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations.Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex.
Product information
Synonym | L3T4; Ly-4 |
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Expression Host | HEK293 cells |
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Tag | C-His |
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Swiss-Prot | |
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Gene ID | |
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Usage guide
Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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Reconstitution | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.