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Recombinant Mouse Latent TGF-beta 1 Protein (RP01167)

Bioactivityvalidated

Publication (1) Datasheet

Overview

Product nameRecombinant Mouse Latent TGF-beta 1 Protein
Catalog No.RP01167
DescriptionRecombinant Mouse Latent TGF-beta 1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu30-Ser390) of mouse TGF-beta 1 (Accession #NP_035707.1) fused with a 8×His tag at the N-terminus.
Bio-ActivityMeasured by its ability to inhibit the IL-4-dependent proliferation of HT-2 mouse T cells. The ED50 for this effect is 0.02-0.1 ng/mL, corresponding to a specific activity of 1.0×107-5.0×107units/mg.
Purity> 95% by SDS-PAGE.
Endotoxin< 0.1 EU/μg of the protein by LAL method.
FormulationLyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
SpeciesMouse
Calculated MW42.11 kDa
SynonymTGF-beta1; Tgfb; Tgfb-1; TGFbeta1; CED; DPD1; LAP; TGFB; TGFbeta; TGFB1
Expression HostHEK293 cells
TagN-His
Swiss-Prot
Gene ID
StorageStore at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
ReconstitutionCentrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile 4 mM HCl. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.

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Discontinued

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