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Recombinant Mouse Leukemia inhibitory factor/LIF Protein (RP01416)

Bioactivityvalidated

Datasheet

Overview

Product nameRecombinant Mouse Leukemia inhibitory factor/LIF Protein
Catalog No.RP01416
DescriptionRecombinant Mouse Leukemia inhibitory factor/LIF Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ser24-Phe203) of mouse LIF (Accession #NP_032527.1) fused with a 6×His,Avi tag at the C-terminus.
Bio-ActivityMeasured in a cell proliferation assay using TF-1 Human erythroleukemic cells. The ED50 for this effect is 35.675-142.7 ng/mL, corresponding to a specific activity of 7.00×104~2.80×104 units/mg.
Purity> 90% by SDS-PAGE.
Endotoxin< 0.1 EU/μg of the protein by LAL method.
FormulationLyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
SpeciesMouse
Calculated MW2.65 kDa
Leukemia inhibitory factor (LIF) is a pleiotropic glycoprotein belonging to the IL-6 family of cytokines. It is involved in growth promotion and cell differentiation of different types of target cells, influence bone metabolism, cachexia, neural development, embryogenesis, and inflammation. LIF has potent proinflammatory properties, being the inducer of the acute phase protein synthesis and affecting cell recruitment into the area of damage or inflammation. LIF is also one of the cytokines that are capable to regulate the differentiation of embryonic stem cells, hematopoietic, and neuronal cells. LIF binds to the specific LIF receptor (LIFR-α) which forms a heterodimer with a specific subunit common to all members of that family of receptors, the GP130 signal-transducing subunit. This leads to the activation of the JAK/STAT and MAPK cascades. Due to its polyfunctional activities, LIF is involved in the pathogenic events and development of many diseases of various origins.
SynonymLIF; CDF; DIA; HILDA; MLPLI; LIF
Expression HostHEK293 cells
TagC-His&Avi
Swiss-Prot
Gene ID
StorageStore at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
ReconstitutionCentrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.

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